{"id":505,"date":"2023-12-06T13:05:37","date_gmt":"2023-12-06T13:05:37","guid":{"rendered":"http:\/\/dira.jecool.net\/?page_id=505"},"modified":"2023-12-15T11:17:17","modified_gmt":"2023-12-15T11:17:17","slug":"vyzkum","status":"publish","type":"page","link":"https:\/\/pitpraha.cz\/?page_id=505&lang=cs","title":{"rendered":"V\u00fdzkum"},"content":{"rendered":"\n
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Vliv elektrick\u00e9ho pole na proteiny<\/h2>\n\n\n\n
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Spektroskopie jako v\u011bdn\u00ed discipl\u00edna se zam\u011b\u0159uje na studium interakce mezi sv\u011btlem a hmotou. Typicky slou\u017e\u00ed jako n\u00e1stroj k anal\u00fdze struktury a dynamiky protein\u016f. Nicm\u00e9n\u011b je zaj\u00edmavou ot\u00e1zkou, zda lze elektromagnetick\u00e9 pole vyu\u017e\u00edt k indukov\u00e1n\u00ed zm\u011bn ve struktu\u0159e nebo dynamice protein\u016f, co\u017e by otev\u0159elo obrovsk\u00fd potenci\u00e1l pro aplikace v medic\u00edn\u011b a nanotechnologii. Ve spolupr\u00e1ci s v\u00fdzkumnou skupinou Dr. Michala Cifry<\/a> z \u00dastavu fotoniky a elektroniky Akademie v\u011bd \u010cesk\u00e9 republiky p\u0159edpov\u00edd\u00e1me mo\u017en\u00e9 \u00fa\u010dinky elektrick\u00e9ho pole na proteiny prost\u0159ednictv\u00edm po\u010d\u00edta\u010dov\u00fdch simulac\u00ed.<\/p>\n<\/div>

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Relevantn\u00ed publikace<\/strong><\/strong><\/div><\/summary>
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Marracino, Paolo, et al. “Tubulin response to intense nanosecond-scale electric field in molecular dynamics simulation.” Scientific reports<\/em> 9.1 (2019): 10477. <\/p>\n\n\n\n

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Pr\u016f\u0161a, Ji\u0159\u00ed, and Michal Cifra. “Molecular dynamics simulation of the nanosecond pulsed electric field effect on kinesin nanomotor.” Scientific Reports<\/em> 9.1 (2019): 19721.<\/p>\n\n\n\n

St\u00e1hnout<\/a><\/div>\n\n\n\n
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Pr\u016f\u0161a, Ji\u0159\u00ed, et al. “Electro-opening of a microtubule lattice in silico.” Computational and Structural Biotechnology Journal<\/em> 19 (2021): 1488-1496.<\/p>\n\n\n\n

St\u00e1hnout<\/a><\/div>\n<\/div><\/details>\n<\/div>\n<\/div>\n<\/div><\/div>\n<\/div>\n<\/div><\/div>\n\n\n\n
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Spektroskopick\u00fd v\u00fdzkum samouspo\u0159\u00e1daj\u00edc\u00edch se struktur<\/h2>\n\n\n\n
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Ve spolupr\u00e1ci se skupinou Prof. Petra Bou\u0159e<\/a> z \u00dastavu Organick\u00e9 Chemie a Biochemie akademie v\u011bd \u010cesk\u00e9 Republiky se zam\u011b\u0159ujeme ve sv\u00e9m v\u00fdzkumu na spektroskopick\u00e9 studium samoorganizuj\u00edc\u00edch se biomolekul\u00e1rn\u00edch struktur. Konkr\u00e9tn\u011b optickou aktivitou t\u011bchto molekul projevuj\u00edc\u00ed se rozd\u00edlnou absorpc\u00ed levo- a pravo-to\u010div\u011b polarizovan\u00e9ho sv\u011btla chir\u00e1ln\u00edmi molekulami. Tato specializovan\u00e1 spektroskopie, zn\u00e1m\u00e1 jako chiroptick\u00e1 spektroskopie, zahrnuje pou\u017eit\u00ed vibra\u010dn\u00edho kruhov\u00e9ho dichroismu (VCD) pro absorpci v infra\u010derven\u00e9 oblasti. V jednom z projekt\u016f je na\u0161e pozornost zam\u011b\u0159ena na zes\u00edlen\u00fd VCD sign\u00e1l fibril. Fibrily jsou vysoce organizovan\u00e9 proteinov\u00e9 agreg\u00e1ty, a jsou p\u0159edm\u011btem na\u0161eho v\u00fdzkumu kv\u016fli jejich p\u0159edpokl\u00e1dan\u00e9 negativn\u00ed roli, kterou hraj\u00ed p\u0159i vzniku neurodegenerativn\u00edch onemocn\u011bn\u00ed, jako jsou Alzheimerova, Parkinsonova, Huntingtonova nemoc nebo prionov\u00e9 encefalopatie.<\/p>\n<\/div><\/div>\n\n\n\n

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Relevantn\u00ed publikace<\/strong><\/div><\/summary>
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Pr\u016f\u0161a, Ji\u0159\u00ed, and Petr Bou\u0159. “Transition dipole coupling modeling of optical activity enhancements in macromolecular protein systems.” Chirality<\/em> 30.1 (2018): 55-64.<\/p>\n\n\n\n

St\u00e1hnout<\/a><\/div>\n\n\n\n
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Kurochka, Andrii, et al. “\u03b1-Synuclein Conformations followed by vibrational optical activity. Simulation and understanding of the spectra.” Physical Chemistry Chemical Physics<\/em> 23.31 (2021): 16635-16645.<\/p>\n\n\n\n

St\u00e1hnout<\/a><\/div>\n<\/div><\/details>\n<\/div>\n<\/div>\n<\/div><\/div>\n<\/div>\n<\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-templates\/template-pagebuilder-full-width.php","meta":{"neve_meta_sidebar":"full-width","neve_meta_container":"contained","neve_meta_enable_content_width":"on","neve_meta_content_width":100,"neve_meta_title_alignment":"","neve_meta_author_avatar":"","neve_post_elements_order":"","neve_meta_disable_header":"","neve_meta_disable_footer":"on","neve_meta_disable_title":"","_themeisle_gutenberg_block_has_review":false,"_ti_tpc_template_sync":false,"_ti_tpc_template_id":"","footnotes":""},"class_list":["post-505","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/pitpraha.cz\/index.php?rest_route=\/wp\/v2\/pages\/505","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pitpraha.cz\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/pitpraha.cz\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/pitpraha.cz\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/pitpraha.cz\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=505"}],"version-history":[{"count":3,"href":"https:\/\/pitpraha.cz\/index.php?rest_route=\/wp\/v2\/pages\/505\/revisions"}],"predecessor-version":[{"id":510,"href":"https:\/\/pitpraha.cz\/index.php?rest_route=\/wp\/v2\/pages\/505\/revisions\/510"}],"wp:attachment":[{"href":"https:\/\/pitpraha.cz\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=505"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}